Cd9 antigen is a glycoprotein expressed on the surface of developing b lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated t lymphocytes and by neurons and glial cells in the peripheral nervous system. Polyclonal antibodies were produced against the segment ala 449sertyrvalserprometleulysgluasnlysalavalproargser 465 located in the pdomain of rpc7 see figure 731. Polyclonal antibodies an overview sciencedirect topics. A functionally relevant conformational epitope on the cd9. Cells infected with herpes simplex virus 1 export to. Monoclonal antibodies mab or moab are antibodies that are made by identical immune cells that are all clones of a unique parent cell. However, polyclonal antibodies in mice can be collected from ascites fluid using any one of a number of ascites producing methodologies. Immunogen description synthesized peptide derived from the internal region of human cd9. Nov 04, 2016 while results between the monoclonal and polyclonal antibodies for each epitope were similar, a greater percentage of reads mapped to their expected region of the genome table 4 for the polyclonal antibody to h3k4me3 34% polyclonal mapping to transcription start sites vs. Pdf antibodies to cd9, a tetraspan transmembrane protein. Cd9 is a 24 kd type iii transmembrane protein also known as tetraspanin, mrp1 and drap24. Polyclonal antibody to detect cd9 in human, mouse, bovine, sheep and rat samples. The antibody has been extensively validated for western blotting using whole cell lysates. Rabbit antihomo sapiens human cd9 polyclonal antibody.
Cd9 antibody polyclonal cd9 antibodywb ihc if elisa. Selected quality suppliers for anti cd9 antibodies. An isolated, synthetic or recombinant antibody, or functional part or functional equivalent thereof, that is specific for an epitope of cd9 comprising at least one amino acid selected from the group consisting of k169, d171, v172 and l173 of the cd9 sequence as depicted in fig. Antigen defined by monoclonal antibody 60229 antibody. Antibodies to cd9, a tetraspan transmembrane protein, inhibit canine distemper virusinduced cellcell fusion but not viruscell fusion article pdf available in journal of virology 7416. Page 1 of 1 iim0117ex 20070830 specificity cd9 p24. Cd9, a major platelet cell surface glycoprotein, is a roca antigen. By means of a polyclonal antibody n1 raised against recombinant swine cd9 protein, we studied the immunohistochemical expression of. The british journal of haematology publishes original research papers in clinical, laboratory and experimental haematology. Tetraspanin29, tspan29, p24, cd9, cd9, mic3, tspan29 targetspecificity this cd9 antibody is generated from rabbits immunized with a klh conjugated synthetic peptide between 115145 amino acids from the central region of human cd9. Polyclonal antisera are difficult to reproduce because of the variety of antibodies made in the polyclonal. The differences between monoclonal and polyclonal antibodies.
Specificity cd9 polyclonal antibody detects endogenous levels of cd9. Commander cd9 anticorps monoclonal et polyclonal pour beaucoup dapplications. As an original manufacturer for its entire catalog of antibodies. We understand much of your research is extremely important to the health of the community. Ex201 cd9 monoclonal antibody overview description purified mouse monoclonal antibody specificity cd9 human properties form liquid. Antibodies several antiintegrin monoclonal antibodies were used in this study, including the anti. The exosome anticd9 antibody recognizes the cd9p24 antigen, a member of the tetraspanin family. Anticd9 antibody produced in rabbit 1 mgml, affinity. Anti cd9 antibody produced in rabbit is suitable for immunohistochemistry at a working dilution of 1. Antibodies to cd9, a tetraspan transmembrane protein, inhibit. Anti cd63 antibody precisionab polyclonal antibody biorad. Platelet activation by cd9 monoclonal antibodies is mediated. Cd9 rabbit antihuman, polyclonal antibody, abnova 400l. Polyclonal antibodies were produced against the prosegment aa 18187 2, against the nterminal segment aa 217233 1,6 or against the cysotolic tail aa 10361051 20.
This lecture explains about polyclonal antibody structure and the production of polyclonal antibodies. Pdf difference between monoclonal and polyclonal antibodies. Cd9 antibody detects endogenous levels of total cd9 protein. References 91 anti cd9 antibody epr2949 see all cd9 primary antibodies. Rabbit anti human cd63 antibody recognizes the cd63 antigen, also known as granulophysin, lysosomeassociated membrane glycoprotein 3, melanomaassociated antigen me491, melanomaassociated. Monoclonal antibodies can have monovalent affinity, in that they bind to the same epitope the part of an antigen that is recognized by the antibody. It also explains the use of polyclonal antibody in biological techniques like immunobloting. Proteintech is committed to ensuring your research continues during the covid19 situation.
Rabbit anti human cd63 antibody detects a band of 3065 kda. The use of these mixed populations of antibodies creates a variety. Cd9 and cd81 interactions and their structural modelling in sperm prior to fertilization. View pdf in international journal of molecular sciences on 19 april 2018 by frolikova, m. It was used as a primary antibody for the immunolocalization of adhesion protein cd9 during a study to investigate the impact of adhesion proteins on skeletal myoblast. The schedules and methodologies used to immunize rabbits and produce polyclonal antibodies will continue to vary dependent upon the immunogen, the adjuvant, and the. Antibody production in chickens is an alternative in vivo technique for polyclonal antibody production. Polyclonal vs monoclonal antibodies pacific immunology.
This cd9 antibody is generated from rabbits immunized with a klh conjugated synthetic peptide between 2151 amino acids of human cd9. Thermo fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation. Alternative splicing results in multiple transcript variants encoding different protein isoforms provided by refseq, apr 2012. Antibody production in chickens offers the advantage of providing a noninvasive means to obtain antibody. Order monoclonal and polyclonal cd9 antibodies for many applications. It is aimed to help labome visitors find the most suited cd9 antibody. Cd9 antibody rabbit polyclonal from proteintech validated in western blot wb, immunohistochemistry ihc, flow cytometry fc, enzymelinked. Rocal, which binds preferentially to rostra1 versus caudal sympathetic ganglia and intercostal nerves. Cd9 on preb cells may play a role in cellcell adhesion. Protein recombinant human cd9 protein tagged ab152262 wb, sdspage, elisa. Cd9 rabbit antihuman, mouse, rat, polyclonal, invitrogen 100. Monoclonal antibody cd9 pn im0117 purified freezedried 0.
Rabbit polyclonal cd9 antibody for elisa, icc, if, ihc, wb. Polyclonal antibodies recognize multiple epitopes on any one antigen. In addition, cd9 may play a role in signal transduction mediated by interaction with low molecular weight gtpbinding proteins. Even in hyperimmune animals, seldom are more than onetenth of the circulating antibodies specific for one antigen. Western blot cd9 polyclonal antibody a1703 western blot analysis of extracts of mouse kidney, using cd9 antibody a1703 at 1. Immunogen this cd9 antibody is generated from klhconjugated synthetic peptide encompassing a sequence within the center region of human cd9. Cd9 antibody was raised in rabbit using synthesized peptide derived from. Informations about anti cd9 antibody stj92147200 you have no items in your shopping cart. Rabbit polyclonal cd9 antibody aa 120165 for facs, if p, ihc p, wb. Tested in western blot wb, immunofluorescence if and immunocytochemistry icc applications. Polyclonal and monoclonal antibody production microbiology. Cd9 rabbit antihuman, mouse, rat, polyclonal, invitrogen 100 l. The product is either no longer available from the supplier or has been delisted at labome. Pdf evidence that monoclonal antibodies against cd9 antigen.
Recombinant anticd9 antibody epr2949 ko tested ab92726. Here we describe that an anticd9 monoclonal antibody mab k41 did not interfere with binding of cdv to cells and uptake of virus. Cd9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. Antigen defined by monoclonal antibody 602 29 antibody. Ex201 cd9 monoclonal antibody overview description purified mouse monoclonal antibody specificity cd9 human properties form liquid appearance colourless buffer phosphate buffered saline pbs with 15 mm sodium azide, ph 7. Polyclonal antibodies pabs are antibodies that are secreted by different b cell lineages within the body whereas monoclonal antibodies come from a single cell lineage. A cd9 peptide according to the invention, or a compound that comprises a cd9 peptide according to the invention, for use as a detection moiety for cd9 specific binding compounds such as antibodies andor immune cells is also herewith provided, as well as a method for determining whether a sample comprises cd9 specific antibodies andor cd9. Purification the antibody was affinitypurified from rabbit antiserum by affinitychromatography using epitopespecific immunogen applications wb ihc if elisa. Cd9 is expressed on early b cells, eosinophils, basophils and activated t cells and is a major component of the platelet cell surface. The main difference between monoclonal and polyclonal antibodies is that.
Detection of cd9 in human platelets by flow cytometry. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it. Systematic comparison of monoclonal versus polyclonal. A monoclonal antibody, by contrast, represents antibody from a single antibody producing b cell and therefore only binds with one unique epitope. Specificity cd9 polyclonal antibody detects endogenous levels of cd9 protein. Cd9 mrp1 polyclonal antibody rsc96 cells probed with\\tcd9\mrp1 polyclonal antibody, unconjugated bs2489r at 0. Polyclonal antibody production will remain an essential research activity and rabbits will continue to serve as one of the primary species used in polyclonal antibody production. The association of cd9 with the gpiibiiia complex is unaffected by a combination of aspirin and adp scavengers sufficient to abrogate anti cd9 monoclonal antibody induced platelet aggregation. Cd9 polyclonal antibody abbkine antibodies, proteins. Polyclonal antibody to detect endogenous levels of cd9 in human, mouse and rat samples. Cd9 rabbit antihuman, mouse, rat, polyclonal, invitrogen. They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Pdf immunohistochemical distribution of the tetraspanin cd9 in. Unlike polyclonal antibodies, which are produced in live animals, monoclonal antibodies are produced in vitro using tissueculture techniques. Rabbit polyclonal antibody raised against synthetic peptide of cd9. Page 1 of 1 iim0117ex 20070830 specificity cd9 p24 antigen is a single transmembrane polypeptide of 24 kda related to the tetraspanin tm4 family.
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